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Image Search Results
Journal: PLoS ONE
Article Title: AS101 Prevents Diabetic Nephropathy Progression and Mesangial Cell Dysfunction: Regulation of the AKT Downstream Pathway
doi: 10.1371/journal.pone.0114287
Figure Lengend Snippet: Mesangial cells were pre-treated as in . Cells treated with HG were treated with different concentrations of AS101 (0.1–5 µg/ml). After 48 h, cell lysates were incubated with pepsin for collagen solubilization for 10–12 days. ELISA assay for the detection of collagen type I was performed on soluble collagen extracts. Results represent mean±SEM of four experiments. *p<0.05 increase vs. starved and mannitol groups. #p<0.05 decrease compared to HG group. Collagen in control cells (30.523 µg/100 mg protein) was normalized to 100%.
Article Snippet:
Techniques: Incubation, Enzyme-linked Immunosorbent Assay